This list only includes recurring characters, otherwise known as supporting characters, which appear frequently from time to time during the series' run, often playing major roles in more than one episode, and those in the main cast are listed below. LGBTQ characters which are guest stars or one-off characters are listed on the pages focusing exclusively on gay (in animation and anime), lesbian (in animation and anime), bisexual (in animation and anime), trans, pansexual, asexual, non-binary, and intersex characters.
The entries on this page are organized alphanumerically by duration dates and then alphabetically by the first letter of a specific series.
A 450-year-old vampire who works part-time at an old public bath, desires the blood of an 18-year-old virgin, and watches over the growth of 15-year-old Rihito Tatsun, son of the bathhouse owners, and attempts to prevent Rihito from losing his virginity.[13]
The leader of the escapees from the ninja village, who has memory-erasing powers, and fled to be with her lover, Yuriko, who lives in the civilian world.[14] She introduces Yuriko to Satoko in the third episode as her significant other. She later gives, in the fourth episode, Satoko tips on getting along with her roommate, Konoha, who is cold toward her, and lets Satoko sleep over with her when Konoha kicks her out.[15] The same episode implies that Yuriko is not her first girlfriend, but her previous partners are so forgettable that she doesn't remember their names, and is freeloading with her girlfriend, while engaging in gambling.[14][16]
Japan
Yuriko
Kuro's lover who is willing to offer moral support to her and her friends, though she repeatedly tries to rein in Kuro's frivolous spending on pachinko and other unnecessary objects.[14][16] Her voice actress, Rumi Okubo, called her a "proper adult woman" who worries about Kuro.[17]
Mona Hussein is the teenage daughter of the Muslim Egyptian-American Hussein family. She is initially a closeted lesbian.[18] She is in a relationship with a white girl named Gina.[19] Mona Hussein and Gina are voiced by bisexual actresses Alia Shawkat and Megan Stalter.
Agent Harrington is a DEA agent. In the second episode, Agent Harrington is revealed to be a lesbian as she falls in love with mycologist Amelia.[21]
United States
Amelia "Mushroom"
Queer
Amelia is a mycologist and Agent Harrington's love interest.[21] She has a son from a prior relationship named Wyatt. Amelia is voiced by queer actress Shannon Woodward.
Lil Kev
March 6, 2025
Darnell
Gay
Darnell and Omar are Uncle Richard Jr.'s friends from prison. They have been in a same-sex relationship ever since they were in prison. Richard is unaware of them being a gay couple.[22]
Sunny Green is the main protagonist of the series. While she dates guys of various species; Sunny was also once in a same-sex relationship with a female robot named Strawberry.[23] In the episode "Steel Ball Sounds Like a Very Dangerous Game", Sunny is shown to be very interested in male and female anthropomorphic animal strippers.[24]
United States
Tulip
In the second episode, a robot girl named Strawberry breaks up with her after she slept with a man named Silken Tofu boy, and because she is overprotective, saying she loves her, but she doesn't feel safe around her, causing Tulip to lose it. She becomes briefly involved with a male spider, which she calls "Rutherford", afterward. He dies after biting Tulip.[23][25] She is also friends with Ladi and Sunny (who had also dated Strawberry in the past).
Ladi Olivia Sanchez IV
Ladi Olivia Sanchez IV is one of Sunny Green's friends. She is both interested in men and women.[24]
In this series, Nico Minoru is portrayed as Peter Parker's best friend. As in the comics, like Runaways, she is bisexual, teasing Peter that she will ask out his crush, Pearl Pangan, if he doesn't do so, and she is, also, a love interest for Peter.[26][27][28]
She meets Mai Oduka, a popular school girl and joins her group of friends, but she pretends to be popular while she is usually a loner and socially awkward. She meets Mai on the rootftop of the school one day and Mai thinks she is going to take her own life, with Renako thinking Mai is her best friend. The following day, Mai confesses that she loves Renako and she suggests that they play a game to see which relationship is more suitable for them, either "best friend" or "lover".[29][30]
Japan
Mai Oduka
A popular school girl who confesses her love to Renako Amaori, after believing she saved Renako from taking her own life, and challenges Renako to a game whether they are lovers or best friends. Mai is committed to the former, while Renako is committed to the latter.[30]
She has a wish to end her own life, after her family dies in an accident, but can't go through with it, and she meets a mermaid, Shiori Oumi, who tells her that her flesh and blood are especially delicious to yōkai. Shiori further declares that she will protect her from other yōkai until she is at her most delicious, at which point she will eat her, a fate which Hinako accepts.[31]
Japan
Shiori Oumi
A mermaid who says she will eat Hinako Yaotose when she's at her "most delicious" and is determined to ensure that Hinako won't die, or kill herself, yet.[31][32][33]
A closeted sixteen-year-old high school student who has a crush on his classmate Aiki Hirose. However, he is acutely introverted and has never spoken to him, only fantasizes about speaking to him. He eventually builds his confidence and grows closer to Hirose,[34] who becomes his good friend.
Japan
Notes
^The pilot episode was released on February 1, 2023 but the series proper was released on March 9, 2025.
^ abcEisenbeis, Richard (May 2, 2025). "Ninja and an Assassin Under One Roof Episode 4". Anime News Network. Archived from the original on May 2, 2025. Retrieved May 2, 2025. Kuro is the epitome of a "deadbeat" girlfriend. She lives at her girlfriend's place, sponges off her financially, and spends her days...gambling...We also see...that past girlfriends [for Kuro] are so disposable to her that she doesn't even remember their names.
^"第四葉 忍者と殺し屋の関係" [Leaf 4 The relationship between ninjas and assassins]. Hamburger/Ninkoro Production Committee (in Japanese). Kadokawa. Archived from the original on May 2, 2025. Retrieved May 2, 2025.
^ abEisenbeis, Richard (April 25, 2025). "Ninja and an Assassin Under One Roof Episode 3". Anime News Network. Archived from the original on April 25, 2025. Kuro likewise has issues getting a job and is basically freeloading off her girlfriend while being tempted into gambling in the hopes of making a quick buck
^"スタッフ" [Staff]. Hamburger/Ninkoro Production Committee (in Japanese). Kadokawa. Archived from the original on April 4, 2025. See the comment "百合子 大久保瑠美" which translates as "Yuriko Okubo Rumi"
