List of restriction enzyme cutting sites: Bsa–Bso

Legend of nucleobases
Code	Nucleotide represented
A	Adenine (A)
C	Cytosine (C)
G	Guanine (G)
T	Thymine (T)
N	A, C, G or T
M	A or C
R	A or G
W	A or T
Y	C or T
S	C or G
K	G or T
H	A, C or T
B	C, G or T
V	A, C or G
D	A, G or T

This article contains a list of the most studied restriction enzymes whose names start with Bsa to Bso inclusive. It contains approximately 90 enzymes. The following information is given:

Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature^[1]^[2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action^[3]^[4].
Source: Organism that naturally produces the enzyme.
Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site^[5]^[6].
Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:

EcoR70I

Whole list navigation

Restriction enzymes

Bsa - Bso

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

BsaI Bacillus stearothermophilus 20241 5' GGTCTC
3' CCAGAG 5' ---GGTCTCN NNNN--- 3'
3' ---CCAGAGNNNNN --- 5' Bso31I, BspTNI, Eco31I

Bsa29I Bacillus sp. 29 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' AagI, BanIII, BavCI, BseCI, BspDI, Bsu15I, BsuTUI, ClaI, BbvAII

BsaAI Bacillus stearothermophilus G668 5' YACGTR
3' RTGCAY 5' ---YAC GTR--- 3'
3' ---RTG CAY--- 5'

BsaBI Bacillus stearothermophilus B674 5' GATN₄ATC
3' CTAN₄TAG 5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'

BsaHI^[7] Bacillus stearothermophilus CPW11 5' GRCGYC
3' CYGCRG 5' ---GR CGYC--- 3'
3' ---CYGC RG--- 5' AcyI, AhaII, AosII, AstWI, AsuIII, BbiII, BstACI, HgiI, PamII

BsaJI Bacillus stearothermophilus J695 5' CCNNGG
3' GGNNCC 5' ---C CNNGG--- 3'
3' ---GGNNC C--- 5'

BsaMI Bacillus stearothermophilus M293 5' GAATGC
3' CTTACG 5' ---GAATGCN --- 3'
3' ---CTTAC GN--- 5' Asp26HI, Asp35HI, Asp36HI, Asp50HI, BsaMI, BsmI, Mva1269I

BsaOI Bacillus stearothermophilus O-122 5' CGRYCG
3' GCYRGC 5' ---CGRY CG--- 3'
3' ---GC YRGC--- 5'

BsaWI Bacillus stearothermophilus W1718 5' WCCGGW
3' WGGCCW 5' ---W CCGGW--- 3'
3' ---WGGCC W--- 5'

BsaXI^[8]

Bacillus stearothermophilus 25B

5' ACN₅CTCC
3' TGN₅GAGG

5' ---ACN₅CTCCN₆NNNN --- 3'
3' ---TGN₅GAGGN₆N NNN--- 5'

BsmCI, BsmDI, BsmXI

BscI Bacillus sp. 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BanIII, BavCI, Bci29I, BdiI, Bli41I, BliAI, LplI, PgaI, SpmI

Bsc4I Bacillus schlegelii 4 5' CCN₇GG
3' GGN₇CC 5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'

Bsc91I Bacillus sp. 5' GAAGAC
3' CTTCTG 5' ---GAAGACNN NNNN--- 3'
3' ---CTTCTGNNNNNN --- 5'

Bsc107I Bacillus schlegelii 107 5' CCN₇GG
3' GGN₇CC 5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'

BscAI Bacillus schlegelii S3 5' GCATC
3' CGTAG 5' ---GCATCNNNN NN--- 3'
3' ---CGTAGNNNNNN --- 5'

BscBI

Bacillus sp. A11

5' GGNNCC
3' CCNNGG

5' ---GGN NCC--- 3'
3' ---CCN NGG--- 5'

AspNI,	BmiI,	BspLI, NlaIV,
PspN4I

BscCI Bacillus sp. 2G 5' GAATGC
3' CTTACG 5' ---GAATGCN --- 3'
3' ---CTTAC GN--- 5' Asp26HI, Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, PctI

BscFI Bacillus sp. JY391 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bfi57I, Bsp143I, BspJI, BtkII, CviAI, Kzo9I, NlaII, SsiBI

Bse1I Bacillus stearothermophilus 1 5' ACTGG
3' TGACC 5' ---ACTGGN --- 3'
3' ---TGAC CN--- 5'

Bse8I Bacillus sp. 8 5' GATN₄ATC
3' CTAN₄TAG 5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'

Bse15I Bacillus sp. 15 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' Ama87I, AvaI, BcoI, BsoBI, BstSI, Eco88I, NspSAI, OfoI

Bse16I Bacillus sp. 16 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AeuI, BseBI, BsiLI, BstNI, BstM6I, BthDI, BthEI, EcoRII, MvaI

Bse17I Bacillus sp. 17 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGI

Bse21I Bacillus sp. 21 5' CCTNAGG
3' GGANTCC 5' ---CC TNAGG--- 3'
3' ---GGANT CC--- 5' AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI

Bse24I Bacillus sp. 24 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGI

Bse64I Bacillus sp. 64 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AspAI, Bse64I, BseT9I, BstPI, EcaI, Eci125I, EcoO65I, NspSAII

Bse118I^[9] Bacillus sp. 118 5' RCCGGY
3' YGGCCR 5' ---R CCGGY--- 3'
3' ---YGGCC R--- 5'

Bse634I 1KNV Bacillus sp. 634 5' RCCGGY
3' YGGCCR 5' ---R CCGGY--- 3'
3' ---YGGCC R--- 5'

BseAI^[10] Bacillus sphaericus 5' TCCGGA
3' AGGCCT 5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5' AccIII, Aor13HI, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, Kpn2I

BseBI^[11] Bacillus stearothermophilus 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AjnI, AorI, Bse17I, Bst1I, BstOI, Bst2UI, EcoRII, MvaI, SleI, ZanI

BseCI^[12]^[13]^[14] Bacillus sp. 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' AagI, BanIII, BavCI, Bsa29I, BspDI, Bsu15I, BsuTUI, ClaI, LcaI

BseDI Bacillus stearothermophilus RFL1434 5' CCNNGG
3' GGNNCC 5' ---C CNNGG--- 3'
3' ---GGNNC C--- 5'

Bse3DI Bacillus stearothermophilus 3D 5' GCAATG
3' CGTTAC 5' ---GCAATGNN --- 3'
3' ---CGTTAC NN--- 5'

BseGI Bacillus stearothermophilus Vs 34-031 5' GGATG
3' CCTAC 5' ---GGATGNN --- 3'
3' ---CCTAC NN--- 5'

BseJI Bacillus stearothermophilus Tsp5 5' GATN₄ATC
3' CTAN₄TAG 5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'

BseKI Bacillus stearothermophilus Ra3-212 5' GCAGC
3' CGTCG 5' ---GCAGCN₇N NNNN--- 3'
3' ---CGTCGN₇NNNNN --- 5' AlwXI, BbvI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I

BseLI Bacillus stearothermophilus LK3-551 5' CCN₇GG
3' GGN₇CC 5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'

BseMI Bacillus stearothermophilus Isl 15-111 5' GCAATG
3' CGTTAC 5' ---GCAATGNN --- 3'
3' ---CGTTAC NN--- 5'

BseMII^[15] Bacillus stearothermophilus Isl 15-111 5' CTCAG
3' GAGTC 5' ---CTCAGN₇NNN --- 3'
3' ---GAGTCN₇N NN--- 5'

BseNI Bacillus sp. N 5' ACTGG
3' TGACC 5' ---ACTGGN --- 3'
3' ---TGAC CN--- 5'

BsePI Bacillus stearothermophilus P6 5' GCGCGC
3' CGCGCG 5' ---G CGCGC--- 3'
3' ---CGCGC G--- 5'

BseQI Bacillus sp. Q 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

BseRI^[16] Bacillus sp. R 5' GAGGAG
3' CTCCTC 5' ---GAGGAGN₇NNN --- 3'
3' ---CTCCTCN₇N NN--- 5'

BseSI^[17] Bacillus stearothermophilus Jo-553 5' GKGCMC
3' CMCGKG 5' ---GKGCM C--- 3'
3' ---C MCGKG--- 5'

BseT9I Bacillus sp. T9 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AcrII, Bse64I, BstEII, BstT9I, EcaI, Eci125I, EcoO65I, NspSAII, PspEI

BseT10I Bacillus sp. T10 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AcrII, AspAI, BstEII, BstT10I, BstPI, EcoO128I, EcoO65I, PspEI

BseXI Bacillus stearothermophilus Ra3-212 5' GCAGC
3' CGTCG 5' ---GCAGCN₇N NNNN--- 3'
3' ---CGTCGN₇NNNNN --- 5' AlwXI, BbvI, BseKI, Bsp423I, Bst12I, Bst71I, BstV1I

BseX3I Bacillus stearothermophilus X3 5' CGGCCG
3' GCCGGC 5' ---C GGCCG--- 3'
3' ---GCCGG C--- 5' AaaI, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII

BseYI Bacillus sp. 2521 5' CCCAGC
3' GGGTCG 5' ---C CCAGC--- 3'
3' ---GGGTC G--- 5'

BseZI Bacillus sp. Z 5' CTCTTC
3' GAGAAG 5' ---CTCTTCN NNN--- 3'
3' ---GAGAAGNNNN --- 5'

BsgI Bacillus sphaericus B922 5' GTGCAG
3' CACGTC 5' ---GTGCAGN₁₂NNNN --- 3'
3' ---CACGTCN₁₂NN NN--- 5'

BshI Bacillus sphaericus 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

Bsh45I

Bacillus sphaericus 45

5' GWGCWC
3' CWCGWG

5' ---GWGCW C--- 3'
3' ---C WCGWG--- 5'

Alw21I, AspHI, Bbv12I, BsiHKAI,

HgiAI,

HpyF46II,

MspV281I

Bsh1236I Bacillus sphaericus RFL1236 5' CGCG
3' GCGC 5' ---CG CG--- 3'
3' ---GC GC--- 5' AccII, BceBI, BspFNI, Bsp50I, Csp68KVI, FauBII, MvnI, SelI

Bsh1285I Bacillus sphaericus RFL1285 5' CGRYCG
3' GCYRGC 5' ---CGRY CG--- 3'
3' ---GC YRGC--- 5'

Bsh1365I Bacillus sphaericus RFL1365 5' GATN₄ATC
3' CTAN₄TAG 5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'

BshFI^[18] Bacillus sphaericus 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

BshGI Bacillus sphaericus 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, PspGI, SspAI

BshKI Bacillus sphaericus 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AspS9I, Bac36I, BavAII, Bsp1894I, BspBII, CcuI, MaeK81II, Pde12I

BshNI Bacillus sphaericus TK-45 5' GGYRCC
3' CCRYGG 5' ---G GYRCC--- 3'
3' ---CCRYG G--- 5' BanI, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI

BshTI

Bacillus sphaericus Jo22-024

5' ACCGGT
3' TGGCCA

5' ---A CCGGT--- 3'
3' ---TGGCC A--- 5'

AgeI, AsiAI,	AsiGI,	CsiAI
CspAI, PinAI

BsiI^[19]^[20] Bacillus sphaericus 5' CACGAG
3' GTGCTC 5' ---C ACGAG--- 3'
3' ---GTGCT C--- 5'

BsiBI^[21] Bacillus sp. 5' GATN₄ATC
3' CTAN₄TAG 5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'

BsiCI Bacillus sp. 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' AcpI, AsuII, Bpu14I, BspT104I, Csp45I, FspII, NspV, PpaAI, SfuI

BsiEI^[22] Bacillus sp. 5' CGRYCG
3' GCYRGC 5' ---CGRY CG--- 3'
3' ---GC YRGC--- 5'

BsiHKAI^[23]

Bacillus stearothermophilus

5' GWGCWC
3' CWCGWG

5' ---GWGCW C--- 3'
3' ---C WCGWG--- 5'

Alw21I, AspHI, Bbv12I, Bsh45I,

Bsm6I,

HgiAI, MspV281I

BsiHKCI Bacillus sp. HKC 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' AquI, BcoI, Bse15I, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAI

BsiKI^[24] Bacillus sp. 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5'

BsiLI Bacillus sp. 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AglI, BseBI, Bse17I, Bst2I, BstNI, Bst38I, Bst100I, EcoRII, MvaI

BsiMI Bacillus sp. 5' TCCGGA
3' AGGCCT 5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5' AccIII, BbvAIII, BlfI, Bsp13I, BspMII, Bsu23I, Kpn2I, PinBII

BsiQI

Bacillus sp.

5' TGATCA
3' ACTAGT

5' ---T GATCA--- 3'
3' ---ACTAG T--- 5'

AbaI, BclI, BspXII,
BstT7I, FbaI, Ksp22I,	ParI

BsiSI^[25] Bacillus sp. 5' CCGG
3' GGCC 5' ---C CGG--- 3'
3' ---GGC C--- 5'

BsiWI Bacillus sp. 5' CGTACG
3' GCATGC 5' ---C GTACG--- 3'
3' ---GCATG C--- 5'

BsiXI Bacillus sp. X 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BscI, BsiXI, Bsp106I, BspDI, Bsu15I, BsuTUI, ClaI, Rme21I

BsiYI^[26] Bacillus sp. 5' CCN₇GG
3' GGN₇CC 5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'

BsiZI Bacillus sp. 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, MaeK81II, Pde12I

BslI Bacillus sp. 5' CCN₇GG
3' GGN₇CC 5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'

BslFI Bacillus stearothermophilus FI 5' GGGAC
3' CCCTG 5' ---GGGACN₈NN NNNN--- 3'
3' ---CCCTGN₈NNNNNN --- 5'

BsmI Bacillus stearothermophilus NUB 5' GAATGC
3' CTTACG 5' ---GAATGCN --- 3'
3' ---CTTAC GN--- 5' Asp26HI, Asp36HI, Asp40HI, BmaHI, BscCI, Mva1269I, PctI

BsmAI

Bacillus stearothermophilus A664

5' GTCTC
3' CAGAG

5' ---GTCTCN NNNN--- 3'
3' ---CAGAGNNNNN --- 5'

Alw26I,	BcoDI, BscQII,	BsoMAI,
BstMAI

BsmBI Bacillus stearothermophilus B61 5' CGTCTC
3' GCAGAG 5' ---CGTCTCN NNNN--- 3'
3' ---GCAGAGNNNNN --- 5'

BsmFI Bacillus stearothermophilus F 5' GGGAC
3' CCCTG 5' ---GGGACN₈NN NNNN--- 3'
3' ---CCCTGN₈NNNNNN --- 5'

BsmSI Bacillus stearothermophilus CP114 5' CCWWGG
3' GGWWCC 5' ---C CWWGG--- 3'
3' ---GGWWC C--- 5'

Bso31I Bacillus stearothermophilus E31 5' GGTCTC
3' CCAGAG 5' ---GGTCTCN NNNN--- 3'
3' ---CCAGAGNNNNN --- 5'

BsoBI 1DC1 Bacillus stearothermophilus JN2091 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' AquI, BcoI, BsiHKCI, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAI

BsoCI Bacillus stearothermophilus AU891 5' GDGCHC
3' CHCGDG 5' ---GDGCH C--- 3'
3' ---C HCGDG--- 5' AocII, BmyI, Bsp1286I, BspLS2I, MhlI, NspII, SduI

BsoFI Bacillus stearothermophilus F66 5' GCNGC
3' CGNCG 5' ---GC NGC--- 3'
3' ---CGN CG--- 5'