List of restriction enzyme cutting sites: T–Z

Main articles: List of restriction enzyme cutting sites and Restriction enzyme
Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

This article contains a list of the most studied restriction enzymes whose names start with T to Z inclusive. It contains approximately 70 enzymes.

The following information is given:

  • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
  • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:  EcoR70I 


Whole list navigation

Restriction enzymes

T

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
TaaI Thermus aquaticus Vn 4-311 5' ACNGT
3' TGNCA 		5' ---ACN   GT--- 3'
3' ---TG   NCA--- 5'
TaiI Thermus aquaticus cs1-331 5' ACGT
3' TGCA 		5' ---ACGT   --- 3'
3' ---   TGCA--- 5'
TaqI Thermus aquaticus 5' TCGA
3' AGCT 		5' ---T   CGA--- 3'
3' ---AGC   T--- 5'
TaqII Thermus aquaticus YTI 5' GACCGA
3' CTGGCT 		5' ---GACCGAN8NNN   --- 3'
3' ---CTGGCTN8N   NN--- 5'
Taq52I Thermus aquaticus YS52 5' GCWGC
3' CGWCG 		5' ---G   CWGC--- 3'
3' ---CGWC   G--- 5' 		AceI, ApeKI, SuiI, TseI
TaqXI Thermus aquaticus 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, MvaI, SleI, SspAI
TasI Thermus aquaticus Vn 4-211 5' AATT
3' TTAA 		5' ---   AATT--- 3'
3' ---TTAA   --- 5'
TatI Thermus aquaticus CBA1-331 5' WGTACW
3' WCATGW 		5' ---W   GTACW--- 3'
3' ---WCATG   W--- 5'
TauI Thermus aquaticus 5' GCSGC
3' CGSCG 		5' ---GCSG   C--- 3'
3' ---C   GSCG--- 5'
TelI Tolypothrix elabens 5' GACNNNGTC
3' CTGNNNCAG 		5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5' 		AspI, AtsI, PflFI, PsyI, Tth111I
TfiI Thermus filiformis 5' GAWTC
3' CTWAG 		5' ---G   AWTC--- 3'
3' ---CTWA   G--- 5'
ThaI Thermoplasma acidophilum 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		Bpu95I, Bsh1236I, Bsp50I, BstFNI, BstUI, Csp68KVI, FalII, FnuDII
TliI Thermococcus litoralis 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, StrI, XhoI
Tru1I Thermus ruber RFL1 5' TTAA
3' AATT 		5' ---T   TAA--- 3'
3' ---AAT   T--- 5'
Tru9I Thermus ruber 9 5' TTAA
3' AATT 		5' ---T   TAA--- 3'
3' ---AAT   T--- 5'
Tru201I Thermus ruber 201 5' RGATCY
3' YCTAGR 		5' ---R   GATCY--- 3'
3' ---YCTAG   R--- 5'
TscI Thermus sp. 491A 5' ACGT
3' TGCA 		5' ---ACGT   --- 3'
3' ---   TGCA--- 5'
TseI Thermus sp. 93170 5' GCWGC
3' CGWCG 		5' ---G   CWGC--- 3'
3' ---CGWC   G--- 5' 		AceI, ApeKI, SuiI, Taq52I
Tsp1I Thermus sp. 1 5' ACTGG
3' TGACC 		5' ---ACTGGN   --- 3'
3' ---TGAC   CN--- 5'
Tsp32I Thermus sp. 32 5' TCGA
3' AGCT 		5' ---T   CGA--- 3'
3' ---AGC   T--- 5'
Tsp32II Thermus sp. 32 5' TCGA
3' AGCT 		5' ---T   CGA--- 3'
3' ---AGC   T--- 5'
Tsp45I Thermus sp. YS45 5' GTSAC
3' CASTG 		5' ---   GTSAC--- 3'
3' ---CASTG   --- 5'
Tsp49I Thermus sp. 5' ACGT
3' TGCA 		5' ---ACGT   --- 3'
3' ---   TGCA--- 5'
Tsp509I Thermus sp. 5' AATT
3' TTAA 		5' ---   AATT--- 3'
3' ---TTAA   --- 5'
TspBI Thermophilic sp. 5' CCRYGG
3' GGYRCC 		5' ---C   CRYGG--- 3'
3' ---GGYRC   C--- 5'
Tsp4CI Thermus sp. 4C 5' ACNGT
3' TGNCA 		5' ---ACN   GT--- 3'
3' ---TG   NCA--- 5'
TspDTI Thermus sp. DT 5' ATGAA
3' TACTT 		5' ---ATGAAN8NNN   --- 3'
3' ---TACTTN8N   NN--- 5' 		 — None on May 2010 —
TspEI Thermus sp. 1E 5' AATT
3' TTAA 		5' ---   AATT--- 3'
3' ---TTAA   --- 5'
Tsp8EI Thermus sp. 8E 5' GCCN5GGC
3' CGGN5CCG 		5' ---GCCNNNN   NGGC--- 3'
3' ---CGGN   NNNNCCG--- 5'
TspGWI Thermus sp. GW 5' ACGGA
3' TGCCT 		5' ---ACGGAN8NNN   --- 3'
3' ---TGCCTN8N   NN--- 5'
TspMI = UthSI Unidentified thermophile 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		AhyI, Cfr9I, EaeAI, EclRI, PaeBI, PspAI, SmaI, XcyI, XmaI, XmaCI
TspRI Thermus sp. R 5' CASTGNN
3' GTSAC 		5' ---CASTGNN   --- 3'
3' ---   GTSACNN--- 5'
Tth111I Thermus thermophilus 111 5' GACNNNGTC
3' CTGNNNCAG 		5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5' 		AspI, AtsI, PflFI, PsyI, TelI
Tth111II Thermus thermophilus 111 5' CAARCA
3' GTTYGT 		5' ---CAARCAN8NNN   --- 3'
3' ---GTTYGTN8N   NN--- 5'
TthHB8I Thermus thermophilus HB8 5' TCGA
3' AGCT 		5' ---T   CGA--- 3'
3' ---AGC   T--- 5'

U

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
Uba4009I Unidentified bacterium A 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AccEBI, AliI, ApaCI, AsiI, BamHI, BnaI, BspAAIII, RspLKII, SolI
Uba153AI Unidentified bacterium 153A 5' CAGCTG
3' GTCGAC 		5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
UbaM39I Unidentified bacterium M39 5' CAGCTG
3' GTCGAC 		5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
UnbI Unidentified bacterium #8 5' GGNCC
3' CCNGG 		5' ---   GGNCC--- 3'
3' ---CCNGG   --- 5' 		AvcI, BavAII, Bce22I, Bsp1894I, Bsu54I, FmuI, NspIV, UnbI
Uur960I Ureaplasma urealyticum 960 5' GCNGC
3' CGNCG 		5' ---GC   NGC--- 3'
3' ---CGN   CG--- 5'

V

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
Van91I Vibrio anguillarum RFL91 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflBI, PflMI
Vha464I Vibrio harveyi 464 5' CTTAAG
3' GAATTC 		5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5'
VneI Vibrio nereis 18 5' GTGCAC
3' CACGTG 		5' ---G   TGCAC--- 3'
3' ---CACGT   G--- 5' 		Alw44I, ApaLI, SnoI
VpaK32I Vibrio parahaemolyticus 4387-61 5' GCTCTTC
3' CGAGAAG 		5' ---GCTCTTCN   NNN--- 3'
3' ---CGAGAAGNNNN   --- 5'
VpaK11AI Vibrio parahaemolyticus 1743 5' GGWCC
3' CCWGG 		5' ---   GGWCC--- 3'
3' ---CCWGG   --- 5' 		Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, SinI, VpaK11BI
VpaK11BI Vibrio parahaemolyticus 1743-1 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		BsrAI, CauI, EagMI, FdiI, HgiBI, HgiJI, SinI, VpaK11AI
VspI Vibrio sp. 343 5' ATTAAT
3' TAATTA 		5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5' 		AseI, AsnI, BpoAI, PshBI, Sru4DI

X

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
XagI Xanthobacter agilis Vs 18-132 5' CCTN5AGG
3' GGAN5TCC 		5' ---CCTNN   NNNAGG--- 3'
3' ---GGANNN   NNTCC--- 5'
XapI Xanthomonas ampelina Slo 51-021 5' RAATTY
3' YTTAAR 		5' ---R   AATTY--- 3'
3' ---YTTAA   R--- 5'
AcsI, ApoI,  CfaI,  FsiI
XbaI[7] Xanthomonas badrii 5' TCTAGA
3' AGATCT 		5' ---T   CTAGA--- 3'
3' ---AGATC   T--- 5'
XcaI Xanthomonas campestris 5' GTATAC
3' CATATG 		5' ---GTA   TAC--- 3'
3' ---CAT   ATG--- 5'
XceI Xanthomonas campestris Ast 40-024 5' RCATGY
3' YGTACR 		5' ---RCATG   Y--- 3'
3' ---Y   GTACR--- 5'
XciI Xanthomonas citri 5' GTCGAC
3' CAGCTG 		5' ---G   TCGAC--- 3'
3' ---CAGCT   G--- 5'
XcmI Xanthomonas campestris 5' CCAN9TGG
3' GGTN9ACC 		5' ---CCANNNNN   NNNNTGG--- 3'
3' ---GGTNNNN   NNNNNACC--- 5'
XcyI Xanthomonas cyanopsidis 13D5 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XmaI, XmaCI
XhoI Xanthomonas holcicola 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		AbrI, BluI, BssHI, PanI, Sau3239I, Sfr274I, TliI, XpaI
XhoII Xanthomonas holcicola 5' RGATCY
3' YCTAGR 		5' ---R   GATCY--- 3'
3' ---YCTAG   R--- 5'
XmaI Xanthomonas malvacearum 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		AhyI, Cfr9I, EaeAI, EclRI, PaeBI, PspAI, TspMI, XcyI, XmaCI
XmaIII Xanthomonas malvacearum 5' CGGCCG
3' GCCGGC 		5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5' 		AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16I
XmaCI Xanthomonas malvacearum C 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		AhyI, Cfr9I, EaeAI, EclRI, PaeBI, Pac25I, PspAI, TspMI, XcyI, XmaI
XmaJI Xanthomonas maltophilia Jo 85-025 5' CCTAGG
3' GGATCC 		5' ---C   CTAGG--- 3'
3' ---GGATC   C--- 5' 		AspA2I, AvrII, AvrBII, BlnI, BspA2I
XmiI Xanthomonas maltophilia Jo 21-021 5' GTMKAC
3' CAKMTG 		5' ---GT   MKAC--- 3'
3' ---CAKM   TG--- 5' 		AccI, FblI
XmnI Xanthomonas manihotis 7AS1 5' GAAN4TTC
3' CTTN4AAG 		5' ---GAANN   NNTTC--- 3'
3' ---CTTNN   NNAAG--- 5' 		Asp700I, BbvAI, MroXI, PdmI
XorII Xanthomonas oryzae 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa16RI, BspCI, EagBI, ErhB9I, MvrI, Ple19I, PvuI, RshI
XpaI Xanthomonas papavericola 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BssHI, MavI, PanI, SauLPII, Sbi68I, Sol10179I, StrI, XhoI
XspI Xanthomonas sp. YK1 5' CTAG
3' GATC 		5' ---C   TAG--- 3'
3' ---GAT   C--- 5'

Y

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
YenI Yersinia enterocolitica 08 A2635 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		Asp713I, BsuBI, CfrA4I, Ecl37kI, Psp23I, PstI, SalPI, SflI, Sst12I

Z

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
ZanI Zymomonas anaerobia 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		ApaORI, BseBI, BspNI, BstNI, Bst2UI, CthII, EcoRII, MvaI, SspAI
ZhoI Zymomonas holcicola 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BbvAII, BscI, BspJI, ClaI, LcaI, PgaI, SpmI, Ssp27144I
ZraI Zoogloea ramigera 11 5' GACGTC
3' CTGCAG 		5' ---GAC   GTC--- 3'
3' ---CTG   CAG--- 5' 		AatII, Ssp5230I
ZrmI Zoogloea ramigera SCA 5' AGTACT
3' TCATGA 		5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5'
Acc113I, AssI,  BmcAI, Bpa34I, 
DpaI, Eco255I, RflFII, ScaI
Zsp2I Zoogloea sp. 2 5' ATGCAT
3' TACGTA 		5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5' 		BfrBI, Csp68KIII, EcoT22I, NsiI, PinBI, Ppu10I, SepI, SspD5II

Notes

  1. ^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
  2. ^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
  3. ^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
  4. ^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
  5. ^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
  6. ^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
  7. ^ Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.
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Prefix: a b c d e f g h i j k l m n o p q r s t u v w x y z 0 1 2 3 4 5 6 7 8 9

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